Description:
Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set by Centers For Disease Control And Prevention
The Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set is a medical device designed by the Centers For Disease Control And Prevention, specifically for the detection of orthopoxvirus DNA in clinical and environmental samples.
The primary benefit of this device resides in its ability to accurately and rapidly identify non-variola orthopoxvirus. This includes the viruses that cause diseases such as Cowpox, Monkeypox, and Vaccinia, which are structurally similar to the Smallpox virus yet less harmful.
The device allows healthcare laboratories to effectively implement diagnostic and epidemiological studies related to orthopoxvirus, helping to control and prevent the spread of these diseases within communities.
Using Real-time PCR (Polymerase Chain Reaction) technology, the primer and probe set provides swift, reliable, highly sensitive, and specific detection, significantly minimizing the likelihood of false-positive or false-negative results. This demonstrates the device's exceptional effectiveness and makes it an integral part of modern disease prevention and control strategies.
In summary, the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set by the Centers For Disease Control And Prevention offers significant advantages, including timely detection, accurate diagnostics, and substantial contributions to public health and safety.
How It Works:
Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set
The
Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set is an advanced sequencing device used in detecting and diagnosing
Orthopoxvirus infections. It employs the principles of real-time Polymerase Chain Reaction (PCR) technology, coupled with the use of specific primers and probes to identify the presence of non-variola Orthopoxvirus DNA sequences within a clinical specimen.
Working Mechanism of the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set
Nucleic Acid Extraction:
The process begins with the extraction of nucleic acid from the clinical specimens. This aims at isolating viral nucleic acids - the primary targets of the PCR process.
Primer and Probe Design:
The primers and probes, which are strands of short, specific sequences of DNA, are uniquely designed to bind to (anneal) and hence identify non-variola Orthopoxvirus DNA.
Real-time PCR:
The device uses
Real-time PCR, a modern technique that amplifies the targeted DNA sequences and simultaneously measures the quantity of product generated throughout the cycling process.
Detection occurs through a fluorescent signal emitted by the PCR product-specific probe. Importantly, the accumulation of the PCR products is directly proportional to the fluorescent signal intensity, allowing for rapid, precise, and sensitive quantification of the original DNA sequences.
Application
This particular device holds great clinical value in the rapid and specific detection of non-variola Orthopoxvirus infections. It aids in conducting efficient epidemiological surveys to trace viral spread—which is pivotal in implementing effective control strategies.
In conclusion, the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set demystifies the early, specific, and sensitive detection of Orthopoxvirus, heralding a new era of prompt and efficient patient management against poxvirus infections. Remember to always refer to the manufacturer's instructions for details on test procedure, interpretation of results, and more.