Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set
Description:
Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set
Manufactured by the Centers for Disease Control and Prevention, the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set is a sophisticated and versatile piece of medical technology designed to facilitate the detection and identification of non-variola orthopoxvirus DNA.
This device is primarily used in diagnostic settings, working as an invaluable tool in the accurate and rapid diagnosis of infections caused by the non-variola orthopoxvirus. These infections can include Cowpox, Monkeypox, and others. By identifying the presence of these viruses, clinicians are able to swiftly administer the appropriate treatment, potentially reducing the morbidity and mortality associated with such infections.
One of the key benefits of this device is its capitalization on real-time PCR (Polymerase Chain Reaction) technology. Real-time PCR allows for the amplification and simultaneous detection or measurement of specific DNA sequences, resulting in faster and more accurate results than traditional PCR methods. This makes it significantly more efficient in a clinical setting, enabling healthcare providers to respond rapidly to patient needs.
Additionally, the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set is specifically tailored to detect orthopoxvirus. This specificity gives it an edge over more generalized tests, offering higher levels of accuracy. Therefore, this device represents a reliable tool in disease control, facilitating prompt and precise responses to potential outbreaks, and enhancing overall public health safety.
Primary Use:
Primary Use of Non-Variola Orthopoxvirus Real-Time PCR Primer and Probe Set
Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set is a crucial medical device with a meaningful role in today's medical world, falling under the category of 'Sequencers'. It has been designed with the core intent of detecting and identifying the presence of Orthopoxvirus DNA.
Functionality
This device is primarily used in laboratories to perform real-time Polymerase Chain Reaction (PCR) on human clinical specimens. It essentially helps in screening and diagnosing Orthopoxvirus infections.
Identification of Non-variola Orthopoxvirus
Orthopoxvirus genus consists of numerous viral species including Vaccinia, Cowpox, and Monkeypox, all of which, although different from Smallpox or Variola virus, can still cause serious human infections. While less common, these infections present health risks and require accurate identification for proper treatment.
Importance of PCR
Real-time PCR technology enables scientists to simultaneously amplify and detect specific DNA sequences, making it a highly effective tool for the detection of pathogenic organisms such as Orthopoxviruses.
How the Device Works
The device uses specific primers and probes designed to recognize and bind to unique areas within the Orthopoxvirus genome. Once bound, the real-time PCR process is initiated, amplifying any present Orthopoxvirus DNA. This allows for rapid and accurate detection and identification of the virus in the patient's specimen – a critical step towards appropriate clinical management.
In conclusion, the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set is a highly significant tool, offering a precise genetic identification method for diagnosing Orthopoxvirus infections.
How It Works:
Non-variola Orthopoxvirus Realtime PCR Primer and Probe Set Overview
As a medical professional, it is essential to have an in-depth understanding of the technicalities and usage of the Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set. This novel medical device comes under the category of 'Sequencers', offering an advanced approach to detect orthopoxviruses in clinical samples swiftly and effectively.
How It Works
The principle behind this technology lies in the Polymerase Chain Reaction (PCR), an extensively used practice to amplify certain DNA segments. Real-time PCR adds a level of quantification and specificity to conventional PCR methods.
The Non-variola Orthopoxvirus Real-time PCR Primer and Probe Set specifically targets a conserved region of DNA that is prevalent in orthopoxviruses but not in variola virus - the cause of smallpox.
Components
The set consists of two main components:
1. Primers: These are short DNA strands that serve as the starting point for DNA synthesis. They bind to the DNA strand at the region of interest – in this case, the orthopoxvirus DNA – and initiates the replication process.
2. Probes: These are DNA strands complementary to the target DNA sequence. They are tagged with a dye and a quencher. When the probe binds to its complement during PCR, the 5’-nuclease activity of the Taq Polymerase enzyme facilitates the separation of the reporter dye from the quencher. This causes the reporter dye to emit light, which is then detected and measured by the real-time PCR machine.
This technology, therefore, not only identifies the presence of the virus but also gives an approximate count of the virus particles in the sample.
Application
The use of this technology is significant in the rapid detection of Orthopoxvirus infections and in distinguishing them from Variola virus, hence crucial in effective disease management and control. It is an instrumental tool that aids in providing swift and efficient patient care by providing definitive diagnostic results in a time-sensitive manner.
Manufacturer:
Centers for Disease Control and Prevention
Status: In Stock
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